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human glioblastoma cell line u 138 (ATCC)

Name: human glioblastoma cell line u 138
Brand: ATCC
Rating: 96 (601 reviews)

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ATCC human glioblastoma cell line u 138
Human Glioblastoma Cell Line U 138, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 601 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 601 article reviews

human glioblastoma cell line u 138 - by Bioz Stars, 2026-02

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Figure 1. Inﬂuence of THC and CBD, administered alone or in combination, on metabolic activity, viability, apoptosis and autophagy in human glioblastoma cells. U251MG and U138MG cells were incubated for 24 h with selected concentrations of THC and CBD or their combination (in a ratio of 1:1) as well as vehicle control. Thereafter, metabolic activity was determined by WST-1 assay (A,B), cell number by crystal violet staining (C,D), caspase-3/-7 activity by ELISA (E,F) and LC3-I to LC3-II conversion by Western blot analysis (G,H). All percentage values shown refer to the vehicle control, which was set to 100%. The data represent mean values ± SEM of n = 10–12 (4 independent experiments in (A–E)), n = 9 (3 independent experiments in F) and n = 3 or n = 4 independent experiments in (G,H). * p ≤0.05, ** p ≤0.01, *** p ≤0.001 vs. corresponding vehicle control, one-way ANOVA with Dunnett’s post hoc test.

Journal: Cancers

Article Title: The Combination of Δ 9 -Tetrahydrocannabinol and Cannabidiol Suppresses Mitochondrial Respiration of Human Glioblastoma Cells via Downregulation of Specific Respiratory Chain Proteins.

doi: 10.3390/cancers14133129

Figure Lengend Snippet: Figure 1. Inﬂuence of THC and CBD, administered alone or in combination, on metabolic activity, viability, apoptosis and autophagy in human glioblastoma cells. U251MG and U138MG cells were incubated for 24 h with selected concentrations of THC and CBD or their combination (in a ratio of 1:1) as well as vehicle control. Thereafter, metabolic activity was determined by WST-1 assay (A,B), cell number by crystal violet staining (C,D), caspase-3/-7 activity by ELISA (E,F) and LC3-I to LC3-II conversion by Western blot analysis (G,H). All percentage values shown refer to the vehicle control, which was set to 100%. The data represent mean values ± SEM of n = 10–12 (4 independent experiments in (A–E)), n = 9 (3 independent experiments in F) and n = 3 or n = 4 independent experiments in (G,H). * p ≤0.05, ** p ≤0.01, *** p ≤0.001 vs. corresponding vehicle control, one-way ANOVA with Dunnett’s post hoc test.

Article Snippet: The human glioblastoma cell line U138MG cell line (#ACC 291; RRID: CVCL_0020) was bought from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany).

Techniques: Activity Assay, Incubation, Control, WST-1 Assay, Staining, Enzyme-linked Immunosorbent Assay, Western Blot

$Figure 3. Inﬂuence of THC and CBD on mitochondrial structure and permeability of human glioblas- toma cells. Representative images from transmission electron microscopy of U251MG (A) and U138MG cells (B) treated for 24 h with vehicle (left) or 2.5 µM THC/CBD (right). The marked struc- tures correspond to mitochondria (M) and autophagosomes (AP), respectively. (C,D) are enlarged views of the outlined areas in (A,B), with yellow arrows indicating the mitochondria marked in the overview images. Mitochondrial density of all mitochondria per image was quantiﬁed as intensity value subtracted from maximal display value (E,F). Data were obtained from 7 images/108 mitochon- dria (vehicle), 7 images/58 mitochondria (THC/CBD, panel E), and 11 images/115 mitochondria (vehicle) or 8 images/74 mitochondria (THC/CBD, panel F), respectively. Cytochrome c (Cyt c) was determined by Western blot analysis in the cytosolic and isolated mitochondrial fraction (G,H). A representative Western blot and the quantitative analysis of n = 3–5 independent experiments are shown. The values correspond to the mean values ± SEM. * p ≤0.05, *** p ≤0.001 vs. corresponding vehicle control, unpaired two-tailed t test.$

Journal: Cancers

doi: 10.3390/cancers14133129

Figure Lengend Snippet: Figure 3. Inﬂuence of THC and CBD on mitochondrial structure and permeability of human glioblas- toma cells. Representative images from transmission electron microscopy of U251MG (A) and U138MG cells (B) treated for 24 h with vehicle (left) or 2.5 µM THC/CBD (right). The marked struc- tures correspond to mitochondria (M) and autophagosomes (AP), respectively. (C,D) are enlarged views of the outlined areas in (A,B), with yellow arrows indicating the mitochondria marked in the overview images. Mitochondrial density of all mitochondria per image was quantiﬁed as intensity value subtracted from maximal display value (E,F). Data were obtained from 7 images/108 mitochon- dria (vehicle), 7 images/58 mitochondria (THC/CBD, panel E), and 11 images/115 mitochondria (vehicle) or 8 images/74 mitochondria (THC/CBD, panel F), respectively. Cytochrome c (Cyt c) was determined by Western blot analysis in the cytosolic and isolated mitochondrial fraction (G,H). A representative Western blot and the quantitative analysis of n = 3–5 independent experiments are shown. The values correspond to the mean values ± SEM. * p ≤0.05, *** p ≤0.001 vs. corresponding vehicle control, unpaired two-tailed t test.

Techniques: Permeability, Transmission Assay, Electron Microscopy, Western Blot, Isolation, Control, Two Tailed Test

$Figure 4. Inﬂuence of THC and CBD, administered alone or in combination, on protein levels of subunits of ETC complexes I (NDUFA9, NDUFB8), II (SDHA, SDHB), III (UQCRC2), IV (COX2, COX4) and V (ATP5A) in human glioblastoma cells. U251MG and U138MG cells were incubated with selected concentrations of THC and CBD or their combination (in a ratio of 1:1) for 24 h. Subsequently, cellular total protein extracts (A,B) or isolated mitochondrial fractions (C–F) were analysed for speciﬁc subunits of different ETC complexes by Western blots. All percentage values shown refer to the vehicle control, which was set to 100%. The values shown in the bar graphs are based on densitometric analyses of blots, whereby the speciﬁc subunits were normalised to β-actin (A,B) or VDAC (C–F). The blots shown are representative. The data in all panels are mean values ± SEM of n = 3–4 independent experiments per group. * p ≤0.05, ** p ≤0.01, *** p ≤0.001 vs. corresponding vehicle control, one-way ANOVA with Dunnett’s post hoc test (A,B) or unpaired two-tailed t test (C–F).$

Journal: Cancers

doi: 10.3390/cancers14133129

Figure Lengend Snippet: Figure 4. Inﬂuence of THC and CBD, administered alone or in combination, on protein levels of subunits of ETC complexes I (NDUFA9, NDUFB8), II (SDHA, SDHB), III (UQCRC2), IV (COX2, COX4) and V (ATP5A) in human glioblastoma cells. U251MG and U138MG cells were incubated with selected concentrations of THC and CBD or their combination (in a ratio of 1:1) for 24 h. Subsequently, cellular total protein extracts (A,B) or isolated mitochondrial fractions (C–F) were analysed for speciﬁc subunits of different ETC complexes by Western blots. All percentage values shown refer to the vehicle control, which was set to 100%. The values shown in the bar graphs are based on densitometric analyses of blots, whereby the speciﬁc subunits were normalised to β-actin (A,B) or VDAC (C–F). The blots shown are representative. The data in all panels are mean values ± SEM of n = 3–4 independent experiments per group. * p ≤0.05, ** p ≤0.01, *** p ≤0.001 vs. corresponding vehicle control, one-way ANOVA with Dunnett’s post hoc test (A,B) or unpaired two-tailed t test (C–F).

Techniques: Incubation, Isolation, Western Blot, Control, Two Tailed Test

Figure 5. Role of cannabinoid-sensitive receptors (A,B), autophagy (C,D) and the proteasome (E,F) in downregulation of the mitochondrial subunit NDUFA9 by the combination of THC and CBD in human glioblastoma cells. U251MG and U138MG cells were incubated with selected concentrations of THC and CBD, their combination (in a ratio of 1:1) or vehicle control for 24 h. The receptor antagonists AM-251, AM-630 and capsazepine (1 µM each; panels (A,B)) were applied to the cells 1 h before the cannabinoids and inhibitors of autophagy (baﬁlomycin A1, 2.5 nM; panels C,D) or proteasome (bortezomib, 10 nM; panels (E,F)) were applied together with the cannabinoids. Antagonists and inhibitors were present throughout the incubation with the cannabinoids. Subsequently, cellular total protein extracts were analysed for NDUFA9 levels by Western blots. All percentage values shown refer to the vehicle control, which was set to 100%. The values shown in the bar charts are based on densitometric analyses of blots, whereby the NDUFA9 levels were normalised to β-actin. The blots shown are representative. The data in all panels are mean values ± SEM of n = 3 independent experiments per group. * p ≤0.05, ** p ≤0.01, *** p ≤0.001 vs. corresponding vehicle control, one-way ANOVA with Bonferroni’s post hoc test.

Journal: Cancers

doi: 10.3390/cancers14133129

Figure Lengend Snippet: Figure 5. Role of cannabinoid-sensitive receptors (A,B), autophagy (C,D) and the proteasome (E,F) in downregulation of the mitochondrial subunit NDUFA9 by the combination of THC and CBD in human glioblastoma cells. U251MG and U138MG cells were incubated with selected concentrations of THC and CBD, their combination (in a ratio of 1:1) or vehicle control for 24 h. The receptor antagonists AM-251, AM-630 and capsazepine (1 µM each; panels (A,B)) were applied to the cells 1 h before the cannabinoids and inhibitors of autophagy (baﬁlomycin A1, 2.5 nM; panels C,D) or proteasome (bortezomib, 10 nM; panels (E,F)) were applied together with the cannabinoids. Antagonists and inhibitors were present throughout the incubation with the cannabinoids. Subsequently, cellular total protein extracts were analysed for NDUFA9 levels by Western blots. All percentage values shown refer to the vehicle control, which was set to 100%. The values shown in the bar charts are based on densitometric analyses of blots, whereby the NDUFA9 levels were normalised to β-actin. The blots shown are representative. The data in all panels are mean values ± SEM of n = 3 independent experiments per group. * p ≤0.05, ** p ≤0.01, *** p ≤0.001 vs. corresponding vehicle control, one-way ANOVA with Bonferroni’s post hoc test.

Techniques: Incubation, Control, Western Blot

$Figure 6. Inﬂuence of THC and CBD, administered alone or in combination, on mitochondrial tran- scription factor A (TFAM) expression (A,B) and heme oxygenase-1 (HO-1) protein levels (C–F) and function (G,H) in human glioblastoma cells. U251MG and U138MG cells were incubated with se- lected concentrations of THC and CBD, their combination (in a ratio of 1:1) or vehicle control for 24 h. In the experiments shown in Panels (G,H), the HO inhibitor SnPPIX (10 µM) was applied to the cells 1 h before the cannabinoids and was present throughout the incubation with the cannabinoids. Following incubation with cannabinoids, total RNA (A,B), cellular total protein (C,D,G,H) or mito- chondrial fractions (E,F) were isolated and analysed for TFAM or HO-1. All percentage values shown refer to the vehicle control, which was set to 100%. The values given in the bar charts are based on RT-PCR (A,B) or densitometric analyses of blots (C,D,G,H), with the gene of interest normalised to PPIA (A,B) and the proteins normalised to β-actin (C,D,G,H). The blots shown are representative. The data in panels (A–D,G,H) are mean values ± SEM of n = 3–5 independent experiments per group. * p ≤0.05, ** p ≤0.01, *** p ≤0.001 vs. corresponding vehicle control, one-way ANOVA with Dunnett’s post hoc test (A–D) or Bonferroni’s post hoc test (G,H).$

Journal: Cancers

doi: 10.3390/cancers14133129

Figure Lengend Snippet: Figure 6. Inﬂuence of THC and CBD, administered alone or in combination, on mitochondrial tran- scription factor A (TFAM) expression (A,B) and heme oxygenase-1 (HO-1) protein levels (C–F) and function (G,H) in human glioblastoma cells. U251MG and U138MG cells were incubated with se- lected concentrations of THC and CBD, their combination (in a ratio of 1:1) or vehicle control for 24 h. In the experiments shown in Panels (G,H), the HO inhibitor SnPPIX (10 µM) was applied to the cells 1 h before the cannabinoids and was present throughout the incubation with the cannabinoids. Following incubation with cannabinoids, total RNA (A,B), cellular total protein (C,D,G,H) or mito- chondrial fractions (E,F) were isolated and analysed for TFAM or HO-1. All percentage values shown refer to the vehicle control, which was set to 100%. The values given in the bar charts are based on RT-PCR (A,B) or densitometric analyses of blots (C,D,G,H), with the gene of interest normalised to PPIA (A,B) and the proteins normalised to β-actin (C,D,G,H). The blots shown are representative. The data in panels (A–D,G,H) are mean values ± SEM of n = 3–5 independent experiments per group. * p ≤0.05, ** p ≤0.01, *** p ≤0.001 vs. corresponding vehicle control, one-way ANOVA with Dunnett’s post hoc test (A–D) or Bonferroni’s post hoc test (G,H).

Techniques: Expressing, Incubation, Control, Isolation, Reverse Transcription Polymerase Chain Reaction

Effect of increasing concentrations of KD87 ( A ), KD85 ( B ), and KD88 ( C ) on viability of various cancer cells. A375 (melanoma; black columns), A431 (skin squamous cell carcinoma; dark grey columns), A549 (non-small cell lung carcinoma; grey columns), U251MG (glioblastoma; light grey columns), or U138MG (glioblastoma; white columns) were incubated for 48 h with the appropriate compounds or vehicle followed by determination of cell viability by WST-1 assay. Percent control represents comparison with vehicle-treated cells (100%) in the absence of test substance. Values are mean ± standard error of the mean (SEM) of n = 15 in all panels, except n = 14 for A375 and A431 cells in panel B and n = 14–15 for A375 cells in panel C, from four (A375, A431) or five (A549, U251MG, U138MG) independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001 vs. vehicle control; one-way ANOVA with Dunnett’s post hoc test.

Journal: Molecules

Article Title: A Sensitive LC-MS/MS Method for the Simultaneous Determination of Two Thia-Analogous Indirubin N -Glycosides and Indirubin-3′-Monoxime in Plasma and Cell Culture Medium

doi: 10.3390/molecules27093031

Figure Lengend Snippet: Effect of increasing concentrations of KD87 ( A ), KD85 ( B ), and KD88 ( C ) on viability of various cancer cells. A375 (melanoma; black columns), A431 (skin squamous cell carcinoma; dark grey columns), A549 (non-small cell lung carcinoma; grey columns), U251MG (glioblastoma; light grey columns), or U138MG (glioblastoma; white columns) were incubated for 48 h with the appropriate compounds or vehicle followed by determination of cell viability by WST-1 assay. Percent control represents comparison with vehicle-treated cells (100%) in the absence of test substance. Values are mean ± standard error of the mean (SEM) of n = 15 in all panels, except n = 14 for A375 and A431 cells in panel B and n = 14–15 for A375 cells in panel C, from four (A375, A431) or five (A549, U251MG, U138MG) independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001 vs. vehicle control; one-way ANOVA with Dunnett’s post hoc test.

Article Snippet: The human glioblastoma cell line U138MG (#ACC 291; RRID: CVCL_0020) and the human lung carcinoma cell line A549 (#ACC 107, RRID:CVCL_0023) were purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany).

Techniques: Incubation, WST-1 Assay, Control, Comparison

Effect of CHIKV Ross and CHIKV Brazil on different cell lines. Comparison of the infectivity/cell damage caused by two CHIKV strains CHIKV Brazil/Ross at increasing MOI. Cell viability was measured in a colorimetric assay (MTS cell viability test) 4dpi . Data are means ± SD of at least three independent experiments with three technical replicates, with 100% corresponding to non-infected cells (Mock). Asterisks indicating the p -values generated in a one-way ANOVA test comparison of non-infected cells with infected cells (green asterisks), and of the different virus strains at the same MOI (grey area and black asterisks). p -values are indicated as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. A Vero-B4 cells (1 × 10 4 cells/well); B A549 cells (1 × 10 4 cells/well); C Huh-7 cells (5 × 10 3 cells/well); D DBTRG cells (1 × 10 4 cells/well); E U138 cells (1 × 10 4 cells/well); F U251 cells (1 × 10 4 cells/well)

Journal: Virus Genes

Article Title: CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251

doi: 10.1007/s11262-022-01892-x

Figure Lengend Snippet: Effect of CHIKV Ross and CHIKV Brazil on different cell lines. Comparison of the infectivity/cell damage caused by two CHIKV strains CHIKV Brazil/Ross at increasing MOI. Cell viability was measured in a colorimetric assay (MTS cell viability test) 4dpi . Data are means ± SD of at least three independent experiments with three technical replicates, with 100% corresponding to non-infected cells (Mock). Asterisks indicating the p -values generated in a one-way ANOVA test comparison of non-infected cells with infected cells (green asterisks), and of the different virus strains at the same MOI (grey area and black asterisks). p -values are indicated as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. A Vero-B4 cells (1 × 10 4 cells/well); B A549 cells (1 × 10 4 cells/well); C Huh-7 cells (5 × 10 3 cells/well); D DBTRG cells (1 × 10 4 cells/well); E U138 cells (1 × 10 4 cells/well); F U251 cells (1 × 10 4 cells/well)

Article Snippet: Vero-B4 cells (ATCC® CCL-81TM) [ ], A549 cells (ATCC® CCL-185TM) [ ], Huh-7 cells (JCRB0403) [ ], the glioblastoma cell line DBTRG-05MG (ATCC® CRL-2020TM) [ ], were obtained from ATCC whilst the human glioblastoma cell lines U138 (aka U-138 MG, ATCC® HTB-16TM) and U251 (aka U-251 MG, ATCC® HTB-17TM; formerly known as U-373 MG) were a gift of R. Brack-Werner, Institute of Virology, German Research Center for Environmental Health (GmbH).

Techniques: Comparison, Infection, Colorimetric Assay, Generated, Virus

Comparison of compound efficacy and toxicity against CHIKV Ross and CHIKV Brazil in the cell lines Vero-B4 and U138. Cells were treated with certain concentrations of HCQ, RBV, or T-1105 and were either infected with CHIKV (efficacy test A and C ) or not (toxicity test B and D ). Four days after infection/treatment, cell survival was determined with MTS. Values are given as percentages in relation to Mock control and are means of three independent experiments each with at least three technical replicates. A Vero-B4 and C U138 cells were infected with CHIKV Ross (white columns) or CHIKV Brazil (grey columns). Statistically significant differences of the compound efficacies between the different virus strains CHIKV Ross and wt CHIKV Brazil in the same cell line were evaluated in a one-way ANOVA test and are indicated by black asterisks. Red asterisks indicate significant (positive) differences between the positive control (black and grey line) and treated, infected cells (same corresponding virus strain and cell line). B and D Compound toxicity in Vero-B4 ( B ) and U138 ( D ) cells. Statistically significant (negative) differences between Mock control (grey bar and green line) and the treated cells (white bars), are indicated by blue asterisks. The number of asterisks indicate p -values as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Journal: Virus Genes

Article Title: CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251

doi: 10.1007/s11262-022-01892-x

Figure Lengend Snippet: Comparison of compound efficacy and toxicity against CHIKV Ross and CHIKV Brazil in the cell lines Vero-B4 and U138. Cells were treated with certain concentrations of HCQ, RBV, or T-1105 and were either infected with CHIKV (efficacy test A and C ) or not (toxicity test B and D ). Four days after infection/treatment, cell survival was determined with MTS. Values are given as percentages in relation to Mock control and are means of three independent experiments each with at least three technical replicates. A Vero-B4 and C U138 cells were infected with CHIKV Ross (white columns) or CHIKV Brazil (grey columns). Statistically significant differences of the compound efficacies between the different virus strains CHIKV Ross and wt CHIKV Brazil in the same cell line were evaluated in a one-way ANOVA test and are indicated by black asterisks. Red asterisks indicate significant (positive) differences between the positive control (black and grey line) and treated, infected cells (same corresponding virus strain and cell line). B and D Compound toxicity in Vero-B4 ( B ) and U138 ( D ) cells. Statistically significant (negative) differences between Mock control (grey bar and green line) and the treated cells (white bars), are indicated by blue asterisks. The number of asterisks indicate p -values as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Techniques: Comparison, Infection, Control, Virus, Positive Control

IC 50 and CC 50 values of different compounds against wt CHIKV Brazil (MOI: 0.355) in Vero-B4 and U138 cells

Journal: Virus Genes

Article Title: CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251

doi: 10.1007/s11262-022-01892-x

Figure Lengend Snippet: IC 50 and CC 50 values of different compounds against wt CHIKV Brazil (MOI: 0.355) in Vero-B4 and U138 cells

Techniques:

IC 50 and CC 50 of HCQ in Vero-B4 and U138 cells. Hydroxychloroquine inhibits CHIKV Brazil -induced cell death in Vero-B4 ( A ) and U138 ( C ) cells in a dose-dependent manner. Cells (1 × 10 4 cells/well) were infected at an MOI of 0.355 and treated with a serial dilution of HCQ. After 4 days, cell death was determined via a colorimetric cell viability assay (MTS). Toxicity assays in Vero-B4 ( B ) and U138 ( C ) cells were performed similarly without infection of the cells. The data represent means ± SD of raw data from at least 3 independent experiments performed with three technical replicates. Normalised fit of dose–response curves was calculated with GraphPad Prism 6 Software

Journal: Virus Genes

Article Title: CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251

doi: 10.1007/s11262-022-01892-x

Figure Lengend Snippet: IC 50 and CC 50 of HCQ in Vero-B4 and U138 cells. Hydroxychloroquine inhibits CHIKV Brazil -induced cell death in Vero-B4 ( A ) and U138 ( C ) cells in a dose-dependent manner. Cells (1 × 10 4 cells/well) were infected at an MOI of 0.355 and treated with a serial dilution of HCQ. After 4 days, cell death was determined via a colorimetric cell viability assay (MTS). Toxicity assays in Vero-B4 ( B ) and U138 ( C ) cells were performed similarly without infection of the cells. The data represent means ± SD of raw data from at least 3 independent experiments performed with three technical replicates. Normalised fit of dose–response curves was calculated with GraphPad Prism 6 Software

Techniques: Infection, Serial Dilution, Viability Assay, Software

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